Saat umur 21 hari, broiler dapat mengatur keseimbangan flora usus. Setelah umur 21 hari tantangan seperti strain, pergantian pakan dan pemberian obat-obatan seperti antibiotik dapat menganggu flora dalam saluran gastrointestinal dan menyebabkan kerugian. Jika saluran usus terkolonisasi dengan mikroba merugikan maka akan berdampak patogen bagi tub
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